Dr. Pam Twigg Lecturer, Chemistry Department Biography My research focus is on the structural and biophysical characterization of proteins, using methods such as x-ray crystallography, NMR spectroscopy, circular dichroism, calorimetry, and fluorescence spectroscopy. In particular, I am interested in the protein-protein interactions and function of proteins related to the pathology of Huntington's Disease, a genetic movement disorder. I’ve been a Research Assistant Professor at The University of Alabama in Huntsville since 2004. Dr. Pam Twigg's Curriculum Vitae Education Ph.D., Molecular Biophysics, Florida State University, 2001 M.S., Biomedical Engineering, University of Alabama at Birmingham, 1986 B.S., Chemical Engineering, Auburn University, 1982 Classes Taught CH 121 - GENERAL CHEMISTRY I CH 301 - ELEMENTARY BIOCHEMISTRY CH 347 - BIOPHYSICAL CHEMISTRY I CH 647 - ADV BIOPHYSICAL CHEMISTRY I Publications Helms, K. M., Wilson, R. C., Ogungbe, I. V., Setzer, W. N., and Twigg, P. D. 2011. "Vitexin Inhibits Polyubiquitin Synthesis by the Ubiquitin-conjugating Enzyme E2-25K." Natural Product Communications, 6(10), 1411-1416. Wilson, R. C., Edmondson, S. P., Flatt, J. W., Helms, K., and Twigg, P. D. 2011. “The E2-25K Ubiquitin-associated (UBA) Domain Aids in Polyubiquitin Chain Synthesis and Linkage Specificity.” Biochem. Biophys. Res. Com., 405(4), 662-666. Twigg, P. D., Lamb, N. E., DuBreuil, R. M., and Zahorchak, R. 2011. “APPLE for the Teacher: Scientists in the Classroom. From Grass Roots to Productive Orchard.” The American Biology Teacher, 73(8), 444-448. Wilson, R. C., Hughes, R. C., Flatt, J. W., Meehan, E. J., Ng, J. D., and Twigg, P. D. 2009. “The crystal structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2).” Acta Cryst., F65, 440-444. Wilson, R. C., Hughes, R. C., Curto, E. V., Ng, J. D., and Twigg, P. D. 2007. “Backbone 1H, 15N, and 13C Resonance Assignments and Secondary Structure of a Novel Protein OGL-20Pt-358 from HyperthermophileThermococcus thioreducens sp. Nov.” Molecules and Cells, 24(3), 437-440. Twigg, P. D., G. Parthasarathy, L. Guerrero, T. M. Logan, and D. L. D. Caspar. 2001. “Disordered to Ordered Folding in the Regulation of Diphtheria Toxin Repressor Activity.” Proc. Natl. Acad. Sci. USA, 98, 11259-64. Wang, G., G. P. Wylie, P. D. Twigg, D. L. D. Caspar, J. R. Murphy, and T. M. Logan. 1999. “Solution Structure and Peptide Binding Studies of the C-Terminal SH3-Like Domain of the Diphtheria Toxin Repressor Protein.” Proc. Natl. Acad. Sci. USA, 96, 6119-6124. Twigg, P. D., G. P. Wylie, G. Wang, D. L. D. Caspar, J. R. Murphy, and T. M. Logan. 1999. “Expression and Assignment of the 1H, 15N, and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor.” J. Biomolecular NMR, 13(2), 197-198. Wardell, M. R., Skinner, R., Carter, D. C., Twigg, P. D., & Abrahams, J.-P. 1997. “Improved diffraction of antithrombin crystals grown in microgravity.” Acta Cryst.D, 53, 622-625. Ho, J.X., E.W. Holowachuk, E.J. Norton, P.D. Twigg, and D.C. Carter. 1993. “X-ray and Primary Structure of Horse Serum Albumin (Equus caballus) at 0.27-nm Resolution.” Eur. J. Biochem., 215, 205-212. Carter, D.C., He, X.-M., Munson, S.H., Twigg, P.D., Gernert, K.M., Broom, M.B., and Miller, T.Y. 1989. “Three-Dimensional Structure of Human Serum Albumin.” Science, 244, 1195-1198.