Dr. Tatyana (Tanya) Sysoeva Assistant Professor, Microbiology, Biological Sciences Department Biography The research program of our lab focuses on horizontal gene transfer (HGT) in complex microbial communities. We analyze which bacteria, when, and by what mechanism exchange genetic material in their native environments. Increasing levels of antibiotic resistance in human bacterial pathogens represent one of the most pressing medical challenges of our time. HGT plays a critical role in spreading of antibiotic resistance genes in bacteria. Currently no solution exists to reverse the antibiotic resistance or prevent it from further spreading via HGT apart from the conservative approach of antibiotics stewardship, that has so far showed limited success in eliminating the resistant strains. Therefore, we study mechanism and regulation of HGT in human pathogenic and commensal bacteria with a far-reaching goal of countering antibiotic resistance spread by modulating DNA exchange. View Dr. Tatyana Sysoeva's Curriculum Vitae Research Website Education Ph.D. Biochemistry and Molecular Biology, The Pennsylvania State University, USA, 2011 M.S.(Hons) Materials Science, Moscow State University, Russia, 2004 B.S. Materials Science, Moscow State University, Russia, 2002 Publications Sysoeva T.A., Kim Y., Rodriguez J., Lopatkin A.J., You L. Growth‐stage‐dependent regulation of conjugation, AIChE Journal, https://doi.org/10.1002/aic.16848 Zhu K., Chen S., Sysoeva T.A., You L. Universal antibiotic tolerance arising from antibiotic-triggered accumulation of pyocyanin in Pseudomonas aeruginosa. PLoS Biol 17(12): e3000573. https://doi.org/10.1371/journal.pbio.3000573 Dai Z., Lee A.J., Roberts S., Sysoeva T.A., Huang S., Dzuricky M., Yang X., Chilkoti A., You L. Versatile biomanufacturing by a hybrid biological-material system. Nat Chem Biol, 15, 1017–1024. Sysoeva T.A. 2017. Assessing heterogeneity in oligomeric AAA+ machines. Cell Mol Life Sci. 74 (6): 1001-1018. Sysoeva T.A., Burton B.M. 2016. A new front for intermicrobial wars. Nat Microbiology, 2, 16254. Lopatkin A.J., Sysoeva T.A., You L. 2016. Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics. BioEssays 38(12):12831290. Lopatkin A.J., Huang S., Smith R., Srimani J., Sysoeva T.A., Bewick S., Karig D., You L. 2016. Antibiotics as a selective driver for conjugation dynamics. Nat Microbiology, 1, 16044. Sysoeva T.A., Bane L.B., Xiao D.Y., Bose B., Chilton S.S., Gaudet R., Burton B.M. 2015. Structural characterization of the late competence protein ComFB from Bacillus subtilis. Biosci Rep, 35(2) Ramsdell T.L., Huppert L.A., Sysoeva T.A., Fortune S.M., Burton B.M. 2015. Linked Domain Architectures Allow for Specialization of Function in the FtsK/SpoIIIE ATPases of ESX Secretion Systems, J Mol Biol, 427(5):1119-1132. Sysoeva T.A., Huppert L.A, Zepeda-Rivera M.A., Burton B.M. 2014. Dimer recognition and secretion by the ESX Secretion System in Bacillus subtilis. Proc Natl Acad Sci U S A, 111(21):7653-7658. Sysoeva T.A., Chowdhury S., Nixon B.T. 2014. Breaking symmetry in multimeric ATPase motors. Cell Cycle, 13(10):1509-1510. Sysoeva T.A., Chowdhury S., Guo L., Nixon B.T. 2013. Nucleotide-induced asymmetry within ATPase activator ring directs sigma54-RNAP interaction and ATP hydrolysis. Genes Dev, 27:2500-2511. The paper was highlighted on the journal cover as “also in this issue”. Sysoeva T.A., Yennawar N., Allaire M., Nixon B.T. 2013. Crystallization and preliminary X-ray analysis of a sigma54-dependent transcription activator NtrC1 from Aquifex aeolicus bound to ground state ATP analog Acta Cryst. F69, 1384-1388. Chen B., Sysoeva T.A.*, Chowdhury S., Guo L., De Carlo S., Hanson J.A., Yang H., and Nixon B.T. 2010. Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase. Structure (Cell Press), 18(11):1420-1430. *Co-first author. The paper was chosen for the journal cover. Publication was accompanied by a comment of Professors Timothy Hoover and Martin Buck. Chen B., Sysoeva T.A., Chowdhury S., Guo L., and Nixon B.T. 2009. ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by co-purification of adenylate kinase of Escherichia coli. FEBS J, 276:807-815. Chen B., Sysoeva T.A., Chowdhury S., and Nixon B.T. 2008. Regulation and action of the bacterial enhancer binding AAA+ ATPases. Biochem. Soc. Transact, 36:89-93.