Dr. Paul Wolf

Department Chair, Biology

Biography

The Wolf lab focuses on the evolution of plants and their genomes. We use a wide range of approaches to explore relationships among plant populations and species. We are currently interested in how homosporous ferns have acquired their typically high chromosome numbers, on average about four times that of flowering plants - Some fern species have over 1000 chromosomes! Patterns of chromosome behavior in ferns can be a reference model for exploring loss of fertility in flowering plants when they undergo genome doubling (polyploidy). Paul G Wolf received his PhD from Washington State University, trained as a post doctoral researcher at University of California, Irvine, and was faculty at Utah State University from 1992 - 2019. He joined the faculty in Biological Sciences at University of Alabama in Huntsville in 2019. The lab is currently accepting masters students in the Biological Sciences Program and PhD students in the Biotechnology Program.

paulwolflab.com/home

Dr. Paul Wolf CV

Education

  • Post doctoral researcher, University of California, Irvine: 1990-1991
  • Ph.D. Botany, Washington State University: 1990
  • M.A. Botany (honors), University of Kansas: 1986
  • B.Sc. Biological Sciences (honors), University of Leicester: 1982

Publications

Peer-reviewed journal articles
* denotes graduate student; & denotes undergrad

  • Wolf PG, Rowe CA, Kinosian SP, Der JP, Lockhart PJ, Shepherd LD, McLenachan PA, Thomson JA. 2019. Worldwide relationships in the fern genus Pteridium (bracken) based on nuclear genome markers. American Journal of Botany 106(10): 1365–1376.
  • Wolf, PG and MS Barker. 2019. Current status and future prospects for fern and lycophyte genomics: introduction to an American Fern Journal special issue. American Fern Journal 109 (3): 177-182.
  • *Robison TA and PG Wolf. 2019. ReFernment: an R package for annotating RNA editing in plastid genomes. Applications in Plant Sciences 7(2): e1216
  • Rowe CA, PG Wolf, RW Lichvar. 2019. Do genetic differences explain the ability of an alkaline shrub to grow in both uplands and wetlands? Western North American Naturalist: 79: 260–269.
  • Ellis, MW, PG Wolf, CA Rowe. 2018. Field identification of Eriogonum corymbosum vars. nilesii and aureum (Polygonaceae). Phytotaxa 382: 293–296.
  • *Robison TA, Grusz AL, Wolf PG, Mower JP, &Fauskee BD, *Sosa K, Schuettpelz E. 2018. Mobile elements shape plastome evolution in ferns. Genome Biology and Evolution 10: 2558-2571.
  • *Lemon JB and PG Wolf. 2018. Genetic differentiation between endemic Eriogonum soredium and its common relative E. shockleyi (Polygonaceae). Systematic Botany 43: 901-909.
  • Rowe, CA, Hauber, DP, Wolf PG. 2018. Genomic variation of introduced Salvinia minima in southeastern United States. Aquatic Botany 151: 38-42. https://doi.org/10.1016/j.aquabot.2018.07.011
  • Li, F-W, Wolf, P.G., et al. 2018. Fern genomes elucidate land plant evolution and cyanobacterial symbioses. Nature Plants 4: 460–472.
  • *Schilling MP, Gompert Z, Li F-W, Windham MD, Wolf PG. 2018. Admixture, evolution, and variation in reproductive isolation in the Boechera puberula clade. BMC Evolutionary Biology 18: 61
  • Wolf, PG, *Robison TA, Johnson MG, Sundue MA, Testo WL, Rothfels CJ. 2018. Target Sequence Capture of Nuclear-Encoded Genes for Phylogenetic Analysis in Ferns. Applications in Plant Sciences 6(5): e1148
  • Cai S, G Chen, Y Wang, Y Huang, DB Marchant, Y Wang, Q Yang, F Dai, A Hills, PJ Franks, E Nevo, DE Soltis, PS Soltis, E Sessa, PG Wolf, D Xue, G Zhang, BJ Pogson, MR Blatt, Z-H Chen. 2017. Evolutionary conservation of ABA signaling for stomatal closure in ferns. Plant Physiology 174: 732-747. doi:10.1104/pp.16.01848
  • The Pteridophyte Phylogeny Group I. 2016. A community-derived classification for extant lycophytes and ferns. Journal of Systematics and Evolution. 54: 563–603.
  • Longhurst ML, DC Coster, PG Wolf, AM *Duffy, H Lee, DT Campbell. 2016. Multi-year professional development grounded in educative curriculum focused on integrating technology with reformed science teaching principles. School Science and Mathematics 116: 430-441
  • olf PG, Rowe CA, Der JP, *Schilling MP, Visger CJ, Thomson J. 2015. Origins and diversity of a cosmopolitan fern genus on an island archipelago. AoB Plants 7: plv118; DOI: 10.1093/aobpla/plv118
  • Wolf PG, Sessa EB, Marchant DB, Li F-W, Rothfels CJ, Sigel EM, Gitzendanner MA, Visger CJ, Banks JA, Soltis DE, Soltis PS, Pryer KM, Der JP. 2015. An exploration into fern genome space. Genome Biology and Evolution 7: 2533-2544.
  • Ellis MW, PG Wolf, S Bardot, &JA Walton, CA Rowe, S Kulpa, and KA Mock. 2015. Further Examination of the Geographic Range of Eriogonum corymbosum var. nilesii (Polygonaceae). Phytotaxa 203: 279–286.
  • Sessa, E.B., J.A. Banks, M.S. Barker, J.P. Der, A.M. *Duffy, S.W. Graham, M. Hasebe, J. Langdale, F.W. Li, D.B. Marchant, K.M. Pryer, C.J. Rothfels, S.J. Roux, M.L. Salmi, E.M. Sigel, D.E. Soltis, P.S. Soltis, D.W. Stevenson, and P.G. Wolf. 2014. Between two fern genomes. GigaScience 3: 15.
  • *Schilling, M., Wolf, P. G., *Duffy, A. M., Rai, H. S., Rowe, C.A., Richardson, B. A., Mock, K. E. 2014. Genotyping-by-sequencing for Populus population genomics: an assessment of genome sampling patterns and filtering approaches. PLoS One 9: e95292.
  • Campbell, T. D., B. A. ZuWallack, D.T, M. Longhurst, B. Shelton, P. G. Wolf. 2014. An examination of the changes in science teaching orientations and technology-enhanced tools for student learning in the context of professional development. International Journal of Science Education. 36: 1815-1848.
  • Davidson, J. B. S. L. Durham, and P. G. Wolf. 2014. Breeding system of the threatened endemic, Primula cusickiana var. maguirei (Primulaceae). Plant Species Biology 29: e55-e63.
  • Rai, H. S., K. E. Mock, B. A. Richardson, R. C. Cronn, K. J. Jayden, J. W. Wright, B. J. Knaus, P. G. Wolf. 2013. Transcriptome characterization and detection of gene expression differences in aspen (Populus tremuloides).  Tree Genetics and Genomes 9: 1031-1041. DOI: 10.1007/s11295-013-0615-y.
  • Mock, K. E., B. A. Richardson, and P. G. Wolf. 2013. Molecular tools and aspen management: a primer and prospectus. Forest Ecology and Management 299: 6-13
  • *Duffy, A. M., Wolf, P. G., Barrow, J., Longhurst, M., Campbell, D.T. & Beckett, H.  2013. Ecological investigations within an interactive plant community simulation. Science Scope 36(8): 42-51.
  • Campbell, D.T., Longhurst, M., *Duffy, A. M, Wolf, P. G., & Shelton, B. E. 2013. Science teaching orientations and technology-enhanced tools for student learning in science. Research in Science Education. 43:20352057
  • Campbell, D.T., Longhurst, M., *Duffy, A. M., Wolf, P. G., & Nagy, R. 2012. Investigating human impact in the environment with faded scaffolded inquiry supported by technologies. Science Activities. 49: 4
  • Mock, K.E., *Callahan, C.M., Islam-Faridi, M.N., Shaw, J.D., Rai, H.S., Sanderson, S.C., Rowe, C.A., Ryel, R.J., Madritch, M.D., *Gardner, R.S., Wolf, P.G., 2012. Widespread triploidy in western North American aspen (Populus tremuloides).  PLoS ONE 7(10): e48406. doi:10.1371/journal.pone.0048406
  • Campbell, D. T, P. G. Wolf, *J. P. Der, E. Packenham, and N. Abd-Hamid. 2012. Scientific inquiry in the genetics laboratory:  biologists and university science teacher educators collaborating to increase engagement in science process. Journal of College Science Teaching 42: 82-89.
  • *Davidson, J. B. and P. G. Wolf. 2011. Natural history of Maguire primrose, Primula cusickiana var. maguirei (Primulaceae). Western North American Naturalist 71: 327 - 337.
  • Banks, J. A.,  T. Nishiyama, M. Hasebe, J. L. Bowman, M. Gribskov, C. dePamphilis, P. G. Wolf et al. 2011. The Selaginella genome identifies changes in gene content associated with the evolution of vascular plants. Science 332: 960-963. DOI: 10.1126/science.1203810.
  • *Der, J. P., M. S. Barker , N. J. Wickett, C. W. dePamphilis, P. G. Wolf. 2011. De novo characterization of the gametophyte transcriptome in bracken fern, Pteridium aquilinum. BMC Genomics 12: 99. doi:10.1186/1471-2164-12-99.
  • Wolf P.G., Der J.P., *Duffy A.M., *Davidson J.B., Grusz A.L., Pryer K.M. 2011. The evolution of chloroplast genes and genomes in ferns. Plant Molecular Biology 76: 251-261. DOI 10.1007/s11103-010-9706-4.
  • Karol, K. G., Arumuganathan, K., Boore, J. L., *Duffy, A. M., Everett, K. D. E., Hall, J. D., &Hansen, S. K., Kuehl, J. V., Mandoli, D. F., Mishler, B. D., Olmstead, R. G., Renzaglia, K. S., Wolf, P. G. 2010. Complete plastome sequences of Equisetum arvense and Isoetes flaccida: implications for phylogeny and plastid genome evolution of early land plant lineages. BMC Evol. Biol. 10: 321.
  • Campbell, D. T., S. W. Wang, H‐Y. Hsu, *A. M. Duffy, and P. G. Wolf. 2010. Learning with Web Tools, Simulations, and Other Technologies in Science Classrooms. Journal of Science Education and Technology 19:505-511.
  • Wolf, P. G., *Roper, J. M., *Duffy, A. M. 2010. The evolution of chloroplast genome structure in ferns. Genome 53: 731-738.
  • Oliver, M. J., A. G. Murdock, B. D. Mishler, J. V. Kuehl, J. L. Boore, D. F. Mandoli, K. D. E. Everett, P. G. Wolf, *A. M. Duffy, K. G. Karol. 2010. Chloroplast genome sequence of the moss Tortula ruralis: gene content, polymorphism, and structural arrangement relative to other green plant chloroplast genomes. BMC Genomics 2010, 11:143
  • Barker, M. S. and P. G. Wolf. 2010. Unfurling fern biology in the genomics age. BioScience 60(3): 177-185.
  • *Ellis, M. W. and P. G. Wolf. 2010. Teaching Species. Evolution: Education and Outreach 3: 89-98.
  • Wolf, P. G., *A. M. Duffy, and *J.M. Roper. 2009. Phylogenetic use of inversions in fern chloroplast genomes. American Fern Journal 99:132-133.
  • nbsp; *Ellis, M. W., *J. M. Roper, &R. Gainer, *J. P. Der, and P. G. Wolf.  2009. The taxonomic designation of Eriogonum corymbosum var. nilesii (Polygonaceae) is supported by AFLP and cpDNA analyses. Systematic Botany 34: 693-703.   
  • *Duffy, A. M., S. A. Kelchner, and P. G. Wolf. 2009. Conservation of selection on matK following an ancient loss of its flanking intron. Gene 438: 17-25.
  • *Der, J. P., J. A. Thomson, &J. K. Stratford & P. G. Wolf. 2009. Global chloroplast phylogeny and biogeography of bracken (Pteridium: Dennstaedtiaceae). American Journal of Botany 96: 1041-1049.
  • *Bjerregaard, L. and P. G. Wolf.  2008. Strong genetic differentiation among neighboring populations of a locally endemic primrose. Western North American Naturalist 68: 66-75.
  • Wickett, N. J. , Y. Zhang, &S. K. Hansen, *J. M. Roper, J. V. Kuehl, S. A. Plock, B. Goffinet, P. G. Wolf, C. W. dePamphilis, Jeffrey L. Boore. 2008. Functional gene losses occur with minimal size reduction in the plastid genome of the parasitic liverwort Aneura mirabilis. Molecular Biology and Evolution 25: 393–401.
  • *Roper, J.M, &S.K. Hansen, P.G. Wolf , K.G. Karol KG, D.F. Mandoli, K.D.E. Everett, J. Kuehl, J.L. Boore. 2007.  The Complete Plastid Genome Sequence of Angiopteris evecta (G. Forst.) Hoffm. (Marattiaceae). American Fern Journal 97 (2): 95-106
  • Smith, A. R., K. M. Pryer, E. Schuettpelz, P. Korall, H. Schneider & P. G. Wolf. 2006. A classification for extant ferns. Taxon 55 (3): 705-731
  • Wolf, P.G, K.G. Karol, D. F. Mandoli, J. Kuehl, K. Arumuganathan, *M. W. Ellis, B. D. Mishler, D. G. Kelch, R. G. Olmstead, J. L. Boore. 2005. The first complete chloroplast genome sequence of a lycophyte, Huperzia lucidula (Lycopodiaceae). Gene 350: 117-128.
  • Wolf, P. G., C. A. Rowe, M. Hasebe. 2004. High levels of RNA editing in a vascular plant chloroplast genome: analysis of transcripts from the fern Adiantum capillus-veneris. Gene 339: 89-97
  • Pryer, K. M., E. Schuettpelz, P. G. Wolf, H. Schneider, A. R. Smith, R. Cranfill,. 2004. Phylogeny and evolution of ferns (monilophytes) with a focus on the early leptosporangiate divergences. American Journal of Botany 91: 1557-1581
  • Nishiyama, T., M. Kugita, R. B. Sinclair, M. Sugita, C. Sugiura, T. Wakasugi, P. G. Wolf, K. Yamada, K. Yoshinaga, and M. Hasebe. 2004. Bryophytes are monophyletic and land plants comprise two extant lineages. Molecular Biology and Evolution, 21 (10): 1813-1819
  • Wolf, P. G., B. Doche, L. Gielly and P. Taberlet. 2004. Genetic structure of Rhododendron ferrugineum at a wide range of spatial scales. Journal of Heredity 95: 301-308
  • Wolf, P. G., C. A. Rowe, R. B. Sinclair, M. Hasebe. 2003. Complete nucleotide sequence of the chloroplast genome from a leptosporangiate fern, Adiantum capillus-veneris L. DNA Research 10 (2): 59-64.
  • *Rowe, D. L., M. T. Murphy, R. C. Fleischer, and P. G. Wolf. 2001. High frequency of extra-pair paternity in Eastern Kingbirds. Condor 103: 845-851.
  •  *Sipes, S. D. and P. G. Wolf.  2001.  Phylogenetic relationships within Diadasia, a group of specialist bees.  Molecular Phylogenetics and Evolution 19: 144-156.
  • Smith, A. R., H. Tuomisto, K. M. Pryer, J. S. Hunt, P. G. Wolf.  2001. Metaxya lanosa, a second species in the genus and fern family Metaxyaceae. Systematic Botany 26: 480-486.
  • &Archibald J.K., P.G. Wolf, V. J. Tepedino, and J. Bair.  2001. Genetic relationships and population structure of the endangered steamboat buckwheat, Eriogonum ovalifolium var. williamsiae (Polygonaceae). American Journal of Botany 88: 608-615.
  • Wolf, P. G.,  D. R. Campbell, N. M. Waser, *S. D. Sipes, T. R. Toler, and &J. K. Archibald. 2001.  Tests of pre-and post-pollination barriers to hybridization between sympatric species of Ipomopsis (Polemoniaceae). American Journal of Botany 88: 213-219.
  • Wolf, P. G., H. Schneider, and T. A. Ranker.  2001. Geographic distributions of homosporous fern taxa: does dispersal obscure evidence of vicariance? Journal of Biogeography 28: 263-270.
  • Pryer, K. M., H. Schneider, A. R. Smith, R. Cranfill, P. G. Wolf, J. S. Hunt, and *S. D. Sipes. 2001. Horsetails and ferns are a monophyletic group and the closest relatives to seed plants. Nature 409: 618-622.
  • Wolf, P. G., *S. D. Sipes, &M. R. White, &M. L. Martines, K. M. Pryer, A. R. Smith, K. Uedo.  1999. Phylogenetic relationships of the enigmatic fern families Hymenophyllopsidaceae and Lophosoriaceae:  evidence from rbcL nucleotide sequences.  Plant Systematics and Evolution 219: 263-270.
  • Soltis, P. S.,  D. E. Soltis, P. G. Wolf, D. L. Nickrent, S-M. Chaw, and R. L. Chapman. 1999. The Phylogeny of land plants inferred from 18S rDNA sequences:  pushing the limits of rDNA signal? Molecular Biology and Evolution 16: 1774 - 1784.
  • *Bobowski, B. R., D. Hole, P. G. Wolf, and L. Bryant. 1999. Identification of roots of woody species using polymerase chain reaction.  Molecular Ecology 8: 485-491.
  • Shelly, J. S., P. Lesica, P. G. Wolf, P. S. Soltis, and D. E. Soltis.  1998. Systematic studies and conservation status of Claytonia lanceolata var. flava (Portulacaceae). Madroño 45: 64-74.
  • Campbell, D. R., N. M. Waser, P. G. Wolf.  1998.  Pollen transfer by natural hybrids and parental species in an Ipomopsis hybrid zone.  Evolution 52: 1602-1611.
  • *Hickerson, L.L. and P.G. Wolf. 1998. Population genetic structure of Arctomecon californica Torrey & Frémont (Papaveraceae) in fragmented and unfragmented habitats. Plant Species Biology 13: 21-33.
  • Kropp, B. R., D. R. Hansen, P. G. Wolf, K. M. Flint, S. V. Thomson. 1997. A study on phylogeny of the dyer's woad rust fungus and other species of Puccinia.  Phytopathology 87: 565-571.
  • Wolf, P. G.  1997.  Evaluation of atpB nucleotide sequences for phylogenetic studies of ferns and other pteridophytes.  American Journal of Botany 84: 1429-1440.
  • *Sipes, S. D. and P. G. Wolf.  1997. Clonal structure and patterns of allozyme diversity in the rare endemic Cycladenia humilis var. jonesii (Apocynaceae). American Journal of Botany 84: 401-409.
  • Wolf, P. G. and R. B. Sinclair.   1997. Highly differentiated populations of the narrow endemic, Maquire primrose (Primula maguirei). Conservation Biology 11: 375-381
  • Wolf, P. G., *R. A. Murray, and *S. D. Sipes.  1997. Species-independent, geographic structuring of chloroplast DNA haplotypes in a montane herb, Ipomopsis (Polemoniaceae).  Molecular Ecology 6: 283-291.
  • Perault, D. R., P. G. Wolf, and T. C. Edwards.  1997. An analysis of genetic variation and population structure in the least (Eutamias minimus) and Uinta (E. imbrinus) chipmunks.  Journal of Mammalogy 78: 134-145.
  • *Lanoue, K. Z., P. G. Wolf, &S. Browning, and E. E. Hood.  1996.  Phylogenetic analysis of restriction site variation in wild and cultivated Amaranthus species (Amaranthaceae).  Theoretical and Applied Genetics 93: 722-732.
  • Hasebe, M. P. G. Wolf et al. 1995.  Fern phylogeny based on rbcL nucleotide sequences.  American Fern Journal 85: 134-181
  • Wolf, P. G. 1995.  Phylogenetic analyses of rbcL and nuclear ribosomal RNA gene sequences in Dennstaedtiaceae.  American Fern Journal 85: 306-327
  • Wolf, P. G. and D. R. Campbell. 1995. Hierarchical analysis of allozymic and morphometric variation in a montane herb, Ipomopsis aggregata (Polemoniaceae). Journal of Heredity 86: 386-394.
  • Wolf, P. G., P. S. Soltis, D. E. Soltis.  1994. Phylogenetic relationships of Dennstaedtioid Ferns: Evidence from rbcL sequence variation.  Molecular Phylogenetics and Evolution 3: 383-392.
  • Wolf, P. G., P. S. Soltis and D. E. Soltis. 1993. Phylogenetic significance of chloroplast DNA restriction site variation in the Ipomopsis aggregata complex and related species (Polemoniaceae).  Systematic Botany 18: 652-662
  • Sheffield, E, P. G. Wolf, F. J. Rumsey, D. J. Robson, T. A. Ranker, S. M. Challinor.  1993.  Spatial distribution and reproductive behavior of a triploid bracken (Pteridium aquilinum) clone in Britain.  Annals of Botany 72: 231-237
  • Wolf, P. G. and P. S. Soltis. 1992.  Estimates of gene flow among populations, geographic races, and species in the Ipomopsis aggregata complex (Polemoniaceae). Genetics 130: 639-647.
  • Soltis, P. S., D. E. Soltis, P. G. Wolf. 1991. Allozymic and chloroplast DNA analysis of polyploidy in Polystichum. I. The origins of P. californicum and P. scopulinum. Systematic Botany 16: 245-256.
  • Wolf, P. G., P. S. Soltis and D. E. Soltis. 1991. Genetic relationships and patterns of allozyme divergence in the Ipomopsis aggregata complex and related taxa (Polemoniaceae). American Journal of Botany 78: 515-526.
  • Wolf, P. G., E. Sheffield and C. H. Haufler. 1991. Estimates of gene flow, genetic substructure and population heterogeneity in bracken (Pteridium aquilinum). Biological Journal of the Linnean Society 42: 407-423.
  • Soltis, P. S., D. E. Soltis and P. G. Wolf. 1990. Allozymic divergence in North American Polystichum (Dryopteridaceae). Systematic Botany 15: 205-215.
  • Wolf, P. G., D. E. Soltis and P. S. Soltis. 1990. Chloroplast DNA and allozymic variation in diploid and autotetraploid Heuchera grossulariifolia (Saxifragaceae) American Journal of Botay 77: 232-244.
  • Wolf, P. G., P. S. Soltis and D. E. Soltis. 1989. Tetrasomic inheritance and chromosome pairing behavior in the naturally occurring autotetraploid Heuchera grossulariifolia (Saxifragaceae). Genome 32: 655-659.
  • Sheffield, E., P. G. Wolf and C. H. Haufler. 1989. How big is a bracken plant? Weed Research 29: 455-460.
  • Sheffield, E., P. G. Wolf, C. H. Haufler, T. A. Ranker and A. C. Jermy. 1989. A re-evaluation of plants referred to as Pteridium herediae (Colmeiros) Löve and Kjellquist. Botanical Journal of the Linnean Society 99: 377-386.
  • Soltis, P. S., D. E. Soltis, P. G. Wolf and J. M. Riley. 1989. Electrophoretic evidence for interspecific hybridization in Polystichum. American Fern Journal 79: 7-13.
  • Wolf, P. G., C. H. Haufler and E. Sheffield. 1988. Genetic variation and mating system of the clonal weed Pteridium aquilinum  (L.) Kuhn (bracken). Evolution 42: 1350-1355.
  • Wolf, P. G., C. H. Haufler and E. Sheffield. 1987. Electrophoretic evidence for genetic diploidy in the bracken fern (Pteridium aquilinum). Science 236: 947-949.
  • Windham, M. D., P. G. Wolf, and T. A. Ranker. 1986. Factors affecting prolonged spore viability in herbarium collections of three species of Pellaea. American Fern Journal 76: 141-148.