Tatyana (Tanya) Sysoeva, Ph.D. Associate Professor, Microbiology Contact 301 Sparkman DriveShelby CenterRoom 369MHuntsville, AL 35899 Campus Map 256.824.6371tatyana.sysoeva@uah.edu Biography The research program of our lab focuses on horizontal gene transfer (HGT) in complex microbial communities. We analyze which bacteria, when, and by what mechanism exchange genetic material in their native environments. Increasing levels of antibiotic resistance in human bacterial pathogens represent one of the most pressing medical challenges of our time. HGT plays a critical role in spreading of antibiotic resistance genes in bacteria. Currently no solution exists to reverse the antibiotic resistance or prevent it from further spreading via HGT apart from the conservative approach of antibiotics stewardship, that has so far showed limited success in eliminating the resistant strains. Therefore, we study mechanism and regulation of HGT in human pathogenic and commensal bacteria with a far-reaching goal of countering antibiotic resistance spread by modulating DNA exchange. Curriculum Vitae Research Website News Education Ph.D. Biochemistry and Molecular Biology, The Pennsylvania State University, USA, 2011 M.S.(Hons) Materials Science, Moscow State University, Russia, 2004 B.S. Materials Science, Moscow State University, Russia, 2002 Expertise Horizontal gene transfer Antibiotic resistance Human urinary microbiome Drug-resistant uropathogenic bacteria Gene exchange in microgravity Planetary protection Recent Publications EF Briggs**, B Ghimire, JA Mayo, MS Kelly, KG Xu, TA Sysoeva. Propagation of antibacterial cold atmospheric pressure plasma through small-bore tubing. PLOS One (accepted) [UAH graduate**] YA Velez Justiniano**, ME Ledford, SL Castro-Wallace, HN Nguyen, TA Sysoeva (2024) More Than a Decade of International Space Station Microbial Sampling in the Environmental Control and Life Support Systems. 53rd International Conference on Environmental Systems Proceedings. (https://ntrs.nasa.gov/citations/20240005682) [UAH graduate**] LM Jeries**, TA Sysoeva, L Karstens, MS Kelly (2024) Synthesis of current pediatric urinary microbiome research. Front. Pediatrics. doi: 10.3389/fped.2024.1396408 [UAH graduate**] MS Kelly, E Dahl, LM Jeries**, TA Sysoeva, L Karstens (2024) Characterization of pediatric urinary microbiome at species-level resolution indicates variation due to sex, age, and urologic history. J.Ped.Urol. https://doi.org/10.1016/j.jpurol.2024.05.016 [UAH graduate**] B Ghimire, EF Briggs**, TA Sysoeva, JA Mayo, KG Xu (2023) Contrasting the Characteristics of Atmospheric Pressure Plasma Jets Operated with Single and Double Dielectric Material: Physicochemical Characteristics and Application to Bacterial Killing. J. Phys D: Applied Physics, 56(8), 085205. doi.org/10.1088/1361-6463/acb602 [UAH graduate**] YAV Justiniano**, DM Goeres, EL Sandvik, BV Kjellerup, TA Sysoeva, JS Harris, S Warnat, M McGlennen, CM Foreman, J Yang, W Li, CD Cassilly, K Lott**, LE HerrNeckar (2023) Mitigation and use of biofilms in space for the benefit of human space exploration. Biofilm, 100102. doi.org/10.1016/j.bioflm.2022.100102. [UAH graduate**] YA Velez Justiniano**, CH Lim*, DS Dunlap, TA Sysoeva (2023) Genome Sequences of Three Common Bacterial Isolates from Wastewater from the Water Processor Assembly at the International Space Station. Microbiology Resource Announcements, e01189-22. doi.org/10.1128/mra.01189-22. [UAH graduate**, UAH undergraduate*] Johnson JA*, Delaney LF*, Ojha V*, Rudraraju M*, Hintze KR*, Siddiqui NY, Sysoeva TA (2022) Commensal urinary lactobacilli inhibit major uropathogens in vitro with heterogeneity at species and strain level. Front. Cell. Infect. Microbiol. https://doi:10.3389/fcimb.2022.870603 [UAH undergraduate*] Starcic Erjavec M., Jeseničnik K., Elam L. P.**, Kastrin A., Predojević L., & Sysoeva T. A. (2022). Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location. Plasmid, 119–120, 102618. https://doi.org/10.1016/J.PLASMID.2022.102618 [UAH graduate**] Karstens L., Siddiqui N.Y., Zaza T.*, Barstad A., Amundsen C.L., Sysoeva T.A. (2021) Benchmarking DNA isolation kits used in analyses of the urinary microbiome. Sci Rep 11, 6186 https://doi.org/10.1038/s41598-021-85482-1 [UAH undergraduate*]