The carbohydrate binding protein Scytovirin has potent antiviral activity. The novel fold imparts specificity for Man4 carbohydrates found on coat glycoprotein of HIV, Ebola, Influenza, and Hepatitis. Biochemical and biophysical studies of the binding and bound complex have lead to structurally engineered mutants that have improved Man4 binding and anti-HIV efficacy. The current research goals are to systematically map mutations of amino acids known to be involved in carbohydrate binding, determine the effects of the number of binding sites on binding and efficacy (multivalency), characterize the structural stability of higher affinity mutants and correlate it to pharmacological activity/longevity, and solve the high-resolution structure of the carbohydrate bound complex. Two Domain Protein High Resolution Solution Structure Carbohydrate Binding Exchange Regimes Fast Exchange Intermediate Exchange Slow Exchange Free Induction Decay: Timing of NMR signal Acquisition 15N Backbone Dynamics as a Function of Bound Carbohydrate
