Dr. Pam Twigg Lecturer, Chemistry Contact 301 Sparkman Drive Materials Science BuildingRoom 225Huntsville, AL 35899 Campus Map 256.824.6935pamela.twigg@uah.edu Biography My research focus is on the structural and biophysical characterization of proteins, using methods such as x-ray crystallography, NMR spectroscopy, circular dichroism, calorimetry, and fluorescence spectroscopy. In particular, I am interested in the protein-protein interactions and function of proteins related to the pathology of Huntington's Disease, a genetic movement disorder. I've been a Research Assistant Professor at The University of Alabama in Huntsville since 2004. Curriculum Vitae Education Ph.D., Molecular Biophysics, Florida State University, 2001 M.S., Biomedical Engineering, University of Alabama at Birmingham, 1986 B.S., Chemical Engineering, Auburn University, 1982 Expertise Biophysical Characterization of Proteins Structural & biophysical characterization of proteins Huntington's Disease research X-Ray Crystallography & NMR Recent Publications Helms, K. M., Wilson, R. C., Ogungbe, I. V., Setzer, W. N., and Twigg, P. D. 2011. "Vitexin Inhibits Polyubiquitin Synthesis by the Ubiquitin-conjugating Enzyme E2-25K." Natural Product Communications, 6(10), 1411-1416. Wilson, R. C., Edmondson, S. P., Flatt, J. W., Helms, K., and Twigg, P. D. 2011. "The E2-25K Ubiquitin-associated (UBA) Domain Aids in Polyubiquitin Chain Synthesis and Linkage Specificity." Biochem. Biophys. Res. Com., 405(4), 662-666. Wilson, R. C., Hughes, R. C., Flatt, J. W., Meehan, E. J., Ng, J. D., and Twigg, P. D. 2009. "The crystal structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2)." Acta Cryst., F65, 440-444. Wilson, R. C., Hughes, R. C., Curto, E. V., Ng, J. D., and Twigg, P. D. 2007. "Backbone 1H, 15N, and 13C Resonance Assignments and Secondary Structure of a Novel Protein OGL-20Pt-358 from HyperthermophileThermococcus thioreducens sp. Nov." Molecules and Cells, 24(3), 437-440. Twigg, P. D., G. Parthasarathy, L. Guerrero, T. M. Logan, and D. L. D. Caspar. 2001. "Disordered to Ordered Folding in the Regulation of Diphtheria Toxin Repressor Activity." Proc. Natl. Acad. Sci. USA, 98, 11259-64. Wang, G., Wylie, G. P., Twigg, P. D., Caspar, D. L. D., Murphy, J. R., and Logan, T. M. 1999. "Solution Structure and Peptide Binding Studies of the C-Terminal SH3-Like Domain of the Diphtheria Toxin Repressor Protein." Proc. Natl. Acad. Sci. USA, 96, 6119-6124 Twigg, P. D., Wylie, G. P., Wang, G., Caspar, D. L. D., Murphy, J. R., and Logan, T. M. 1999. "Expression and Assignment of the 1H, 15N, and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor." J. Biomolecular NMR, 13(2), 197-198. Carter, D. C., Wright, B. S., Miller, T. Y., Chapman, J., Twigg, P. D., Keeling, K. M., Moody, K., White, M., Click, J., Ruble, J. R., Ho, J. X., Adcock-Downey, L., Bunick, G., and Harp, J. 1999. "Diffusion-controlled crystallization apparatus for microgravity (DCAM): flight and ground-based applications." J. Crystal Growth, 196, 602-609. Carter, D. C., Wright, B. S., Miller, T. Y., Chapman, J., Twigg, P. D., Keeling, K. M., Moody, K., White, M., Click, J., Ruble, J. R., Ho, J. X., Adcock-Downey, L., Dowling, T., Chang, C.-H., Ala, P., Rose, J., Wang, B. C., Declerq, J.-P., Evrard, C., Rosenberg, J., Wery, J.-P., Clawson, D., Wardell, M., Stallings, W., and Stevens, A. 1999. "PCAM: a multi-user facility-based protein crystallization apparatus for microgravity." J. Crystal Growth, 196,610-622. Carter, D. C., Lim, K., Ho, J. X., Wright, B. S., Twigg, P. D., Miller, T. Y., Chapman, J., Keeling, K. M., Ruble, J. R., Vekilov, P. G., Thomas, B. R., Rosenberger, F., and Chernov, A. A. 1999. "Lower dimer impurity incorporation may result in higher perfection of HEWL crystals grown in microgravity. A case study." J. Crystal Growth, 196, 623-637.