Dr. Pam Twigg

Lecturer, Chemistry

Contact

301 Sparkman Drive
Materials Science Building
Room 225
Huntsville, AL 35899
Campus Map

256.824.6935
pamela.twigg@uah.edu

Biography

My research focus is on the structural and biophysical characterization of proteins, using methods such as x-ray crystallography, NMR spectroscopy, circular dichroism, calorimetry, and fluorescence spectroscopy. In particular, I am interested in the protein-protein interactions and function of proteins related to the pathology of Huntington's Disease, a genetic movement disorder.

I've been a Research Assistant Professor at The University of Alabama in Huntsville since 2004.

Curriculum Vitae

Education

  • Ph.D., Molecular Biophysics, Florida State University, 2001
  • M.S., Biomedical Engineering, University of Alabama at Birmingham, 1986
  • B.S., Chemical Engineering, Auburn University, 1982

Expertise

  • Biophysical Characterization of Proteins
  • Structural & biophysical characterization of proteins
  • Huntington's Disease research
  • X-Ray Crystallography & NMR

Recent Publications

  • Helms, K. M., Wilson, R. C., Ogungbe, I. V., Setzer, W. N., and Twigg, P. D. 2011. "Vitexin Inhibits Polyubiquitin Synthesis by the Ubiquitin-conjugating Enzyme E2-25K." Natural Product Communications, 6(10), 1411-1416.

  • Wilson, R. C., Edmondson, S. P., Flatt, J. W., Helms, K., and Twigg, P. D. 2011. "The E2-25K Ubiquitin-associated (UBA) Domain Aids in Polyubiquitin Chain Synthesis and Linkage Specificity." Biochem. Biophys. Res. Com., 405(4), 662-666.

  • Wilson, R. C., Hughes, R. C., Flatt, J. W., Meehan, E. J., Ng, J. D., and Twigg, P. D. 2009. "The crystal structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2)." Acta Cryst., F65, 440-444.

  • Wilson, R. C., Hughes, R. C., Curto, E. V., Ng, J. D., and Twigg, P. D. 2007. "Backbone 1H, 15N, and 13C Resonance Assignments and Secondary Structure of a Novel Protein OGL-20Pt-358 from HyperthermophileThermococcus thioreducens sp. Nov." Molecules and Cells, 24(3), 437-440.

  • Twigg, P. D., G. Parthasarathy, L. Guerrero, T. M. Logan, and D. L. D. Caspar. 2001. "Disordered to Ordered Folding in the Regulation of Diphtheria Toxin Repressor Activity." Proc. Natl. Acad. Sci. USA, 98, 11259-64.

  • Wang, G., Wylie, G. P., Twigg, P. D., Caspar, D. L. D., Murphy, J. R., and Logan, T. M. 1999. "Solution Structure and Peptide Binding Studies of the C-Terminal SH3-Like Domain of the Diphtheria Toxin Repressor Protein." Proc. Natl. Acad. Sci. USA, 96, 6119-6124

  • Twigg, P. D., Wylie, G. P., Wang, G., Caspar, D. L. D., Murphy, J. R., and Logan, T. M. 1999. "Expression and Assignment of the 1H, 15N, and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor." J. Biomolecular NMR, 13(2), 197-198.

  • Carter, D. C., Wright, B. S., Miller, T. Y., Chapman, J., Twigg, P. D., Keeling, K. M., Moody, K., White, M., Click, J., Ruble, J. R., Ho, J. X., Adcock-Downey, L., Bunick, G., and Harp, J. 1999. "Diffusion-controlled crystallization apparatus for microgravity (DCAM): flight and ground-based applications." J. Crystal Growth, 196, 602-609.

  • Carter, D. C., Wright, B. S., Miller, T. Y., Chapman, J., Twigg, P. D., Keeling, K. M., Moody, K., White, M., Click, J., Ruble, J. R., Ho, J. X., Adcock-Downey, L., Dowling, T., Chang, C.-H., Ala, P., Rose, J., Wang, B. C., Declerq, J.-P., Evrard, C., Rosenberg, J., Wery, J.-P., Clawson, D., Wardell, M., Stallings, W., and Stevens, A. 1999. "PCAM: a multi-user facility-based protein crystallization apparatus for microgravity." J. Crystal Growth, 196,610-622.

  • Carter, D. C., Lim, K., Ho, J. X., Wright, B. S., Twigg, P. D., Miller, T. Y., Chapman, J., Keeling, K. M., Ruble, J. R., Vekilov, P. G., Thomas, B. R., Rosenberger, F., and Chernov, A. A. 1999. "Lower dimer impurity incorporation may result in higher perfection of HEWL crystals grown in microgravity. A case study." J. Crystal Growth, 196, 623-637.