Pattern Specific Isotopic Labeling

• PSAL Phenylalanine
• PSAL Ubiquitin Spectrum 

Pattern Specific Phenylalanine Labeling

Cα, Cγ, Cε pattern specifically labeled phenylalanine was produce (inset in left panel). Recombinant ubiquitin was expressed after supplementing with Cα, Cγ, Cε labeled phenylalanine.  A broadband ¹³C-HSQC was collected from the purified protein, with only side chain ¹Hε-¹³Cε and backbone ¹Hα-¹³Cα resonances from the two phenylalanine residues in ubiquitin present (left). An aromatic selective version of the same experiment shows no carbon-carbon coupling (right). All data collected at 500 MHz.