Pattern Specific Isotopic Labeling
- PSAL Phenylalanine
- PSAL Ubiquitin Spectrum
Cα, Cγ, Cε pattern specifically labeled phenylalanine was produce (inset in left panel). Recombinant ubiquitin was expressed after supplementing with Cα, Cγ, Cε labeled phenylalanine. A broadband 13C-HSQC was collected from the purified protein, with only side chain 1Hε-13Cε and backbone 1Hα-13Cα resonances from the two phenylalanine residues in ubiquitin present (left). An aromatic selective version of the same experiment shows no carbon-carbon coupling (right). All data collected at 500 MHz.
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