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Dr.
Luis Rogelio Cruz-Vera
Assistant Professor
Department of Biological Sciences
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Visit our
lab
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Molecular Biology, Microbiology
Translation and Gene expression regulation
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Our goal is to understand how biomolecules can modulate the ribosome function to influence gene expression, metabolism and cellular phenotype.
Translation elongation can be controlled by diverse mechanisms since a substantial number of different factors and events are involved in the process. Despite the diversity, the elongation regulatory mechanisms keep two common and essential characteristics: 1) the translated sequence itself plays an important role in the regulatory process; and 2) the inhibition, or delay in the elongation due to a regulated event can lead to the accumulation of the involved translating peptidyl-tRNA. The objective of our research is to identify genes subject to translation elongation regulation in several model organisms detecting peptidyl-tRNAs involved in the gene expression under relevant cell growth conditions. Also, determine the molecular mechanism(s) responsible for these translational regulation examples. We expect to produce a gene-expression profiling technology to analyze in vivo other important phenomena related to the accumulation of peptidyl-tRNA such as: the action of antibiotic translation inhibitors, codon translation efficiency, coupling translation-protein folding, among other processes.
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Selected Publications
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- Cruz-Vera, L. R., Gong, M. and Yanofsky, C. (2008). Physiological effects of anti-TRAP protein activity and tRNA(Trp) charging on trp operon expression in Bacillus subtilis. J. Bacteriol. 190:1937-1945
- Gong, M., Cruz-Vera, L. R. and Yanofsky, C. (2007). Ribosome recycling factor and release factor 3 action promotes TnaC-peptidyl-tRNA drop off and relieves ribosome stalling during tryptophan induction of tna oepron expression in Escherichia coli. J. Bacteriol. 189:3147-3155
- Cruz-Vera, L. R., Rajagopal, S., Squires, C. and Yanofsky, C. (2005). Features of ribosome-peptidyl-tRNA interactions essential for tryptophan induction of tna operon expression. Mol. Cell. 19:333-343
- Cruz-Vera, L. R., Magos-Castro, M. A., Zamora-Romo, E. and Guarneros, G. (2004). Ribosome stalling and peptidyl-tRNA drop-off during translation delay at AGA codons. Nucleic Acids Res. 18:4462-4468
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