Dr. Roy Magnuson

Associate Professor, Biological Sciences

Contact

301 Sparkman Drive
Shelby Center
Room 369K
Huntsville, AL 35899
Campus Map

256.824.6094
magnusr@uah.edu

Biography

I am interested in addictive genetic elements and particularly in the plasmid addiction operon from the P1 plasmid of Escherichia. This operon encodes two small proteins: a stable, 126 amino acid toxin and an unstable 73 amino acid antidote. While the plasmid is present, the antidote is expressed in sufficient quantities to bind and neutralize the toxin. Upon plasmid loss, the continuing degradation of the antidote by the host-encoded ClpXP protease unveils the toxin, which then kills the plasmid-free segregant. Current research is focused on the structure and function of antidote and toxin, and on the regulatory circuits that govern the level of antidote and the ratio of antidote and toxin.

Dr. Roy Magnuson's Curriculum Vitae


Education

  • Ph.D., Biology, Massachusetts Institute of Technology, 1995
  • B.A., Integrated Science, Northwestern University, 1985
  • B.A., Biochemistry, Northwestern University, 1985

Expertise

  • Plasmid Host Interactions

Recent Publications

  • Garcia-Pino, A., Y. Sterckx, R.D. Magnuson and R. Loris, 2013. "Type II Toxin- Antitoxin Loci: The phd/doc family". In: Gerdes, K., Ed., 2013. Prokaryotic Toxin-Antitoxins, Springer-Verlag, Berlin & Heidelberg pp.157-176. Garcia-Pino, A., Balasubramanian, S., Wyns, L., Gazit, E., De Greve, H., Magnuson, R.D., Charlieer, D., van Nuland, N.A.J., Loris, R., 2010. "Allostery and Intrinsic Disorder Mediate Transcriptional Regulation by Conditional Cooperativity" Cell. 142(1):101-11.

  • Garcia-Pino, A., Balasubramanian, S., Wyns, L., Gazit, E., De Greve, H., Magnuson, R.D., Charlieer, D., van Nuland, N.A.J., Loris, R., 2010. "Allosteryand Intrinsic Disorder Mediate Transcriptional Regulation by Conditional Cooperativity" Cell. 142(1):101-11

  • Garcia-Pino, A., Dao-Thi, M. H., Gazit, E., Magnuson, R. D., Wyns, L., and Loris, R. 2008. Crystallization of Doc and the Phd-Doc toxin-antitoxin complex. Acta Crystallogr Sect F Struct Biol Cryst Commun 64, 1034-1038.

  • Garcia-Pino A, Christensen-Dalsgaard M, Wyns L, Yarmolinsky M, Magnuson RD, Gerdes K, Loris R. 2008. "Doc of Prophage P1 Is Inhibited by Its Antitoxin Partner Phd through Fold Complementation" Journal of Biological Chemistry 283(45):30821-7.

  • Chen Y., Kay Holtman C., Magnuson R.D., Youderian P.A. and Golden S.S. 2008. "The complete sequence and functional analysis of pANL, the large plasmid of the unicellular freshwater cyanobacterium Synechococcus elongatus PCC 7942" Plasmid 59(3):176-92.

  • Magnuson, R.D., 2007. "Hypothetical Functions of Toxin-Antitoxin Systems" J Bacteriol 189(17): 6089-6092.

  • Zhao, Xueyan and Magnuson, R. D. 2005. Percolation of the Phd Repressor- Operator Interface. Journal of Bacteriology, 187:1901-1912.

  • McKinley, James Estle and Magnuson, R. D. 2005. Characterization of the Phd Repressor-Antitoxin Boundary. Journal of Bacteriology 187:765-770.

  • Smith, J. A., and Magnuson, R. D. 2004. Modular Organization of the Phd Repressor/Antitoxin Protein. Journal of Bacteriology 186:2692-2698. Magnuson, R. and Yarmolinsky, M. B. 1998. Corepression the P1 Addiction Operon by Phd and Doc. Journal of Bacteriology 180:6342-6351.

  • Magnuson, R., Lehnherr, H., Mukhopadhyay, G. and Yarmolinsky, M. B. 1996. Autoregulation of the Plasmid Addiction Operon of Bacteriophage P1. Journal of Biological Chemistry 271:18705-18710.

Affiliate Faculty

Hudson Alpha Institute for Biotechnology

Dr. Devin Absher

Research Faculty

Dr. Gregory Barsh

Research Faculty

Dr. Josh Clevenger

Research Faculty

Dr. Sara Cooper

Research Faculty

Dr. Gregory Cooper

Research Faculty

Dr. Jane Grimwood

Research Faculty

Dr. Jian Han

Research Faculty

Dr. Alex Harkess

Research Faculty

Dr. Neil Edward Lamb

Research Faculty

Dr. Elaine Lyon

Research Faculty

Dr. Richard Myers

Research Faculty

Dr. Kankshita Swaminathan

Research Faculty