Dr. Joseph D. Ng

Professor, Biological Sciences Coordinator,
BSE Program


301 Sparkman Drive
Shelby Center
Room 369J
Huntsville, AL 35899
Campus Map



The goal of our laboratory is to elucidate the structure and function of proteins involved in the passage of genetic information. We are developing and utilizing the combined techniques of Molecular Biology, Protein Chemistry, and X-ray and Neutron Crystallography to decipher the molecular mechanism and action of specific hyperthermophilic proteins. Strategies and tools for the rapid construction of new protein complexes are valuable for biotechnology applications. Our studies include the design and engineering of macromolecular systems for DNA replication using gene synthesis and structural biology tools.

Dr. Joseph D. Ng's Curriculum Vitae


  • Ph.D., Biochemistry, University of California, Los Angeles, 1992
  • B.S., Biochemistry, University of California, Los Angeles, 1985


  • Crystallization
  • Structural Biology
  • Structure/Function of Extremeophilic Proteins
  • Synthetic Biology and Engineering
  • Synthetic Biology

Recent Publications

  • Havlickova, P., Brinsa, V., Brynda, J., Pachl, P., Prudnikova, T., Mesters, J.R., Kascakova, B., Kuty, M., Pusey, M.L., Ng, J.D., Rezacova, P. and Smatanova, I.K.(2019). A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity. Acta Cryst. D75, 743-752

  • Ng, J.D., Baird, J.K., Coates, L., Garcia-Ruiz, J.M., Hodge, T.A. and Huan, S. (2015). Large volume protein crystal growth for Neutron Macromolecular Crystallography. Acta Crystallogr Sect F Struct Biol Cryst Commun. 71:358-370.

  • Pusey, M., Barcena, J., Morris, M., Singhal, A., Yuan, Q. and Ng, J.D. (2015). Trace fluorescent labeling for protein crystallization. Acta Crystallogr Sect F Struct Biol Cryst Commun. 71:806-814.

  • Coates, L., Tomanicek, S., Shrader, T., Weiss, K., Ng, J.D., Juttner, P. and Ostermann, A. (2014). Cryogenic neutron protein crystallography: routine methods and potential benefits. Journal of Applied Crystallography.47:1431-1434.

  • Ng, J.D., Dowell, J.J., Kar, A.K., Hansen, K., Thundat, T. and George, M.A. (2013). Measurement of temperature induced unfolding of DNA hairpins by microcantilever sensors. Open Journal of Applied Biosensor. 2:78-82.

  • Tomanicek, S.J.,Standaert, R.F., Weiss, K.L., Ostermann, A., Schrader, T.E., Ng, J.D. Coates L. (2013). Neutron and X-ray crystal structures of a perdeuterated enzyme inhibitor complex reveal the catalytic proton network of the Toho-1 ?-lactamase for the acylation reaction. J. Biol. Chem. 288(7):4715-4722.

  • Hughes, R.C., Coates, L., Blakeley, M.P., Tomanicek, S.J., Langan, P., Kovalesvsky, A., García-Ruiz, J.M. and Ng, J.D.(2012). Inorganic pyrophophatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction. Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1482-1487.

  • Chu, X.Q., Gajapathy, M., Weis, K., Mamontov, E., Ng, J.D. and Coates, L. (2012). Dynamic Behavior of Oligomeric Inorganic Pyrophosphatase Explored by Quasielastic Neutron Scattering. The Journal of Physical Chemistry 116:9917-9921.

  • Tomanicek, S.J., Hughes, R.C., Ng, J.D. and Coates, L. (2010). Structure of the endonuclease IV homologue from Thermotoga maritima in the presence of active-site divalent metal ions. Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1003-1012.

  • Kantardjieff, K.A., Lind, C. Ng, J.D. and Santarsiero, B.(2010). Efforts to enhance coverage of crystallography in United States secondary education. J. Applied Crystallography 43, 1181–1188.

Affiliate Faculty

Hudson Alpha Institute for Biotechnology

Dr. Devin Absher

Research Faculty

Dr. Gregory Barsh

Research Faculty

Dr. Josh Clevenger

Research Faculty

Dr. Sara Cooper

Research Faculty

Dr. Gregory Cooper

Research Faculty

Dr. Jane Grimwood

Research Faculty

Dr. Jian Han

Research Faculty

Dr. Alex Harkess

Research Faculty

Dr. Neil Edward Lamb

Research Faculty

Dr. Elaine Lyon

Research Faculty

Dr. Richard Myers

Research Faculty

Dr. Kankshita Swaminathan

Research Faculty