Dr. Gordon MacGregor

macgregor g

Assistant Professor

Epithelial Physiology
Gastrointestinal Fluid Secretion and Absorption
Renal and Electrolyte Physiology
Electromyography and Muscle Research
Role of Aquaporins in Hydrocephalus

Office: Shelby Center Room 369H
Phone: 256.824.4925
Email: gm0009@uah.edu 

Research Description

The goal of our laboratory is to understand the body's regulation of water and electrolyte balance. Disorders of water balance such as diarrhea (dehydration), and hyponatremia (water toxicity) can be rapidly fatal, and we seek novel, cheap and affordable pharmaceutical cures. The techniques we use are Ussing chambers, patch clamp and modern molecular and biophysical techniques to work on mouse models of disease.

Selected Publications

Heather M. Jones, Mark A. Bailey, Catherine J. Baty, Gordon G. MacGregor, Colin A. Syme, Kirk L. Hamilton, Daniel C. Devor. (2007). An NH2-Terminal Multi-basic RKR Motif is required for the ATP-dependent regulation of hIK1. Channels 1(2): e1-e12.

Matthew A. Bailey, Alessandra Cantone, Q. Yan, Gordon G. MacGregor, Qiang Leng, J.B.O. Amorim Tong Wang, Steven C. Hebert, Gerhard Giebisch and Gerhard Malnic. (2006). Mechanism of urinary potassium excretion in the ROMK-deficient mouse model of type II Bartter's syndrome. Kidney International 70: 51-59.

Qiang Leng*, Gordon G. MacGregor*, Ke Dong, Gerhard Giebisch and Steven C. Hebert (2006). Subunit-subunit interactions are critical for proton sensitivity of ROMK – evidence in support of an intermolecular gating mechanism. Proc Natl Acad Sci U S A. 103: 1982-1987.

Qiang Leng, Kristopher T. Kahle, Jesse Rinehart, Gordon G. MacGregor, Frederick H. Wilson, Cecilia M. Canessa, Richard P. Lifton and Steven C. Hebert. (2006). WNK3, a kinase related to genes mutated in hereditary hypertension with hyperkalemia, regulates the K+ channel ROMK1 (Kir1.1). J. Physiol. 571: 275-286.

O'Connell AD, Leng Q, Dong K, MacGregor GG, Giebisch G, Hebert SC. (2005). Related Articles, Phosphorylation-regulated endoplasmic reticulum retention signal in the renal outer-medullary K+ channel (ROMK). Proc Natl Acad Sci U S A. 102: 9954-9.

Dong, K., L-Q. Tang, G. G. MacGregor, Q. Leng and S. C. Hebert. (2005). Novel Nucleotide Binding-Sites in ATP-Sensitive Potassium channels Formed at Gating Interfaces. EMBO J. 24: 1318-1329.

Kahle KT*, MacGregor GG*, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP. Paracellular Cl- permeability is regulated by WNK4 kinase: insight into normal physiology and hypertension. Proc Natl Acad Sci U S A. 2004 101(41):14877-82

Kahle, K. T., F. H. Wilson, Q. Leng, M. D. Lalioti, A. D. Connell, K. Dong, A. K. Rapson, G. G. MacGregor, G. Giebisch, S. C. Hebert, R. P. Lifton. (2003). WNK4 regulates the balance between renal NaCl reabsorption and K+ secretion. Nature Genetics. 35: 372-376.

Dong, K., L-Q. Tang, G. G. MacGregor, S. C. Hebert. (2002). Localization of the ATP/PIP2-binding site to a 39 amino acid region of the carboxy-terminus of the ATP-regulated K+ channel Kir1.1. J Biol Chem. 277(51):49366-73.

MacGregor G. G., K. Dong, C. G. Vanoye, L. Tang, G. Giebisch, S. C. Hebert. (2002). Nucleotides and phospholipids compete for binding to the C terminus of KATP channels. Proc. Natl. Acad. Sci. USA. 99: 2726-2731.

Vanoye, C. G. *, G. G. MacGregor*, K. Dong, L-Q. Tang, A. S. Buschmann, A. E. Hall, M. Lu, G. Giebisch and S. C. Hebert. (2002). The carboxyl termini of K(ATP) channels bind nucleotides. J. Biol. Chem. 277: 23260-23270.

Baines, D. L., G. G. MacGregor, P. J. Kemp. (2001). Fatty acid modulation and sequence identity of fetal guinea pig alveolar type II cell amiloride-sensitive Na+ channel. Biochem. Biophys. Res. Commun. 288: 727-735.

Dong, K., J. Xu, C. G. Vanoye, R. Welch, G. G. MacGregor, G. Giebisch, S. C. Hebert. (2001). An amino acid triplet in the NH2 terminus of rat ROMK1 determines interaction with SUR2B. J Biol. Chem. 276: 44347-44353.

Riochet, D. F. L., R, Mohammad-Panah, S. C. Hebert, G. G. MacGregor, I. Baró, G. Guihard, D. Escande. (2001). Inactivating properties of recombinant ROMK2 channels expressed in mammalian cells. Biochem Biophys Res Commun. 286: 376-380.

Leipziger, J, G. G. MacGregor, G. J Cooper, J. Xu, S. C. Hebert and G. Giebisch. (2000). PKA site mutations of ROMK2 channels shift the pH-dependence to more alkaline values. Am. J. Physiol. 279: F919-F926.

M. Lu, G. G. MacGregor, W. Wang and G. Giebisch. (2000). Extracellular ATP inhibits the small-conductance K channel on the apical membrane of the cortical collecting duct from mouse kidney. J. Gen. Physiol. 116: 299-310.

G. G. MacGregor, J. Z. Xu, C. M. McNicholas, G. Giebisch and S. C. Hebert. (1998). Partially active channels produced by PKA site mutation of the cloned renal K+ channel ROMK2 (Kir1.2). Am. J. Physiol. 275: F415-F422.

C. M. McNicholas, G. G. MacGregor, L. D. Islas, Y. Yang, S. C. Hebert and G. Giebisch. (1998). pH-dependent modulation of the cloned renal K+ channel, ROMK. Am. J. Physiol. 275: F972-F981.

G. G. MacGregor, R. E. Olver, P. J. Kemp (1994). Amiloride-sensitive Na+ channels in fetal type II pneumocytes are regulated by G proteins. Am. J. Physiol. 267: L1-L8.