Imaging the Chemistry of Biological Materials using Synchrotron X-Ray & Infrared Microscopy
Dr. Lisa M. Miller
Photon Sciences Directorate, National Synchrotron Light Source I & II, Brookhaven National Laboratory, Upton, NY USA
Departments of Chemistry & Biomedical Engineering Stony Brook University, Stony Brook, NY USA
Synchrotron light sources are facilities that produce bright beams of broadband light from x-rays to infrared wavelengths. These beams are well-suited for synchrotron-based spectroscopic microscopy techniques, which have grown in popularity over recent years especially in the biological sciences. For example, x-ray fluorescence microscopy (XFM) can be used to probe the elemental distribution, concentration, and speciation of elements in a specimen. As a complement, Fourier transform infrared microscopy (FTIRM) is sensitive to the organic composition of materials, distinguishing components such as proteins, lipids, nucleic acids, and polysaccharides. And for both methods, the high brightness of the synchrotron source enables micro- to nanoscale spatial resolution and sub-ppm detection sensitivity.
The National Synchrotron Light Source at Brookhaven National Laboratory (Long Island, NY) is a national user facility that provides scientists access to a wide range of synchrotron-based microscopy facilities. In this presentation, this facility will be described along with a range of examples that utilize XFM and/or FTIRM to image the chemistry of biological materials including biopolymers, plants, microbes, and mammalian cells and tissues.
April 14, 2014
Shelby Center room 050 at 4:00 pm
Everyone is welcomed.
Complete seminar schedule.